Part:BBa_K4307003:Design

Bxb1 integrase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 180
  Illegal BamHI site found at 558
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 973
  Illegal AgeI site found at 1333
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We successfully expressed the integrase from bacteriophage in bacteria, and the results of the fluorescence assay showed that integrase could have obvious inversion effect, but the change of Ct values shows that the action time of Bxb1 integrase was a little bit long.

Source

E.coli